Fluorescence In Situ Hybridization (FISH)

Submitted: Saturday 31st of July 2010 05:13:12 PM
Submitted by: egf
Language: English
Educational levels: qc1, qc2, qc3

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Abstract

This resource contains materials from advanced courses of cytogenetics and it is especially focused on Fluorescence In Situ Hybridization (FISH).

Resources

Bauman and colleagues were the first to describe a fluorescent in situ hybridisation (FISH) approach in which a fluorophore was chemically coupled to an RNA probe enabling quick and direct visualisation of hybridised nucleic acids. Now, more then 25 years later, FISH has become a routine method in ...
Bauman and colleagues were the first to describe a fluorescent in situ hybridisation (FISH) approach in which a fluorophore was chemically coupled to an RNA probe enabling quick and direct visualisation of hybridised nucleic acids. Now, 25 years later, FISH has become a routine method in clinical d ...
Over the last years, a multitude of different multicolor-FISH technologies have been developed (for reviews see Fauth and Speicher 2001 or Speicher and Carter 2005). These multicolor technologies can be used for a broad range of diagnostic and research applications. Some of these multicolor technolo ...
Over the last years, a multitude of different multicolor-FISH technologies have been developed (for a reviews see Fauth and Speicher 2001 or Speicher and Carter 2005). These multicolor technologies can be used for a broad range of diagnostic and research applications. Some of these multicolor techno ...
A lecture about Fiber FISH
Fluorescence In Situ Hybridizaion (FISH) is a powerful tool to identify chromosomal aberrations for diagnosis as well as for prognostic stratification of leukemias and lymphomas. Both interphase and metaphase FISH may be applied to investigate numerical and structural chromosome changes. Interphase ...
A lecture on FISH technology in leukemia.
A lecture about FISH in leukemias by C. Mecucci
The term “Fiber-FISH” was first used by Florijn et al (1995) in a review to describe the various high resolution FISH techniques designed with the production of DNA/chromatin fibers on a slide. Fiber FISH is a cytogenetic laboratory technique in which FISH is done on chromosomes that have been ...
Fluorescence In Situ Hybridizaion (FISH) is a powerful tool to identify chromosomal aberrations for diagnosis as well as for prognostic stratification of leukemias and lymphomas. Both interphase and metaphase FISH may be applied to investigate numerical and structural chromosome changes. Interphase ...
A lecture about FISH in hematological malignancies: the Workshop by C.T. Strolazzi in 2007, 9th course in molecular cytogenetics and DNA microarray
Cancer is a multistep process in which more than one specific gene (among proto-oncogenes, tumor-suppressor genes and mutator genes) undergoes mutational events that could alter the normal expression pattern. It is well known that regulatory or structural alterations of cellular oncogenes have been ...
The purpose of this work was to adapt the recently described centromere-specific multicolour (cenM-) FISH technique to human meiotic cells, and evaluate the usefulness of this multiplex fluorescence method for karyotyping human synaptonemal complex (SC), previously analysed by immunocytogenetic ap ...
A lecture on new perspectives in fluorescence microscopy

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Keywords

abnormal, acrocentric, affected, alpha, Analysis of the entire coding region: Sequence analysis, antibody, antigen binding fragment, Array genomic hybridization, association, autosomal, autosome, bacterial artificial chromosome, band, banding, base, base pair, bivalent, blastomere, break-point cluster, candidate gene, carrier, cell, cell line, centimorgan, centromere, chiasma, chromatin, chromosome, chromosome painting, chromosome walking, clinical genetics, clone, cloning, Complementary DNA, complementary strand, constitutional, correlation, cosmid, Cytogenetic Abnormality, Cytogenetic Analysis, cytogenetics, degenerate oligonucleotide-primed pcr, deletion, deoxyribonucleic acid, diakinesis, dna microarray, dna probe, domain, duplication, enhancer, euchromatin, exon, expression, FISH-interphase, FISH-metaphase, fixed, fluorescent dye, fluorescent in situ hybridization, frequency, gene, gene amplification, gene cloning, gene expression, genetic mapping, genetics, genome, genomics, gm, heredity, heterochromatin, heterogeneity, heterozygote, homogeneously staining region, homologous, homologous chromosome, hybrid, hybridization, immunohistochemistry, in situ hybridization, index case, insertion, interphase, interphase cytogenetics, interstitial deletion, intron, inversion, isolated, karyotype, kilobase, linkage, locus, marker chromosome, maternal, meiosis, meiotic prophase I, metaphase, microarray, microrna, mismatch repair mechanism, monosomy, mosaicism, Multicolor FISH, oncogene, p1-derived artificial chromosome, pachytene, plasmid, point mutation, polymerase chain reaction, power, prevalence, probe, promoter, prophase, protein, proto-oncogene, rearrangement, reciprocal translocation, recombinant, recombination, reverse transriptase pcr, ribonucleic acid, ring chromosome, satellite, satellite dna, segregation, sensitivity, sequence, sex chromosome, sex-determining region of the y, single nucleotide polymorphism, somatic cell hybrid, specie, specificity, Spectral Karyotyping, subtelomeric region, survival, synaptonemal complex, syndrome, target dna, telomere, transcript, translocation, trisomy, tumor suppressor gene, uniparental disomy, untranslated region, variant, variation, vector, y-chromosome, yeast artificial chromosome

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