Comparative Genomic Hybridisation (CGH)

Submitted: Saturday 31st of July 2010 05:18:37 PM
Submitted by: egf
Language: English
Educational levels: expert, qc1, qc2, qc3

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Abstract

This resource contains materials from advanced courses of cytogenetics and it is especially focused on Comparative Genomic Hybridisation (CGH).

Resources

Characterizing of Chromosomal Aberrations in Cancer Cells Genomic instability is the hallmark of cancer (Lengauer et al, 1998). As cancer cells develop, genomic aberrations accumulate. These aberrations include large DNA rearrangements such as chromosome loss or duplication or the translocation of ...
The experience of over two years in utilizing array comparative genomic hybridization (array CGH) for clinical genetic diagnosis will be presented. Over 2500 blood samples have been analyzed and a pilot study for prenatal diagnosis has been completed. A published summary of the development of an e ...
The experience of over two years in utilizing array comparative genomic hybridization (array CGH) for clinical genetic diagnosis will be presented. Over 2500 blood samples have been analyzed and a pilot study for prenatal diagnosis has been completed. A published summary of the development of an ea ...
Array -CGH is nowadays the method of choice for studying DNA copy number changes. However, the lack of commercial availability of the arrays is a disadvantage1. Until now, BAC and cDNA clones have been obtained from, for example, the Sanger Center, BACPAC Resources, InVitrogen and Research Genetics. ...
Array -CGH is nowadays the method of choice for studying DNA copy number changes. However, the lack of commercial availability of the arrays is a disadvantage1. Until now, BAC and cDNA clones have been obtained from, for example, the Sanger Center, BACPAC Resources, InVitrogen and Research Genetics. ...
Molecular karyotyping or genome wide array CGH enables the detection of chromosomal copy number variations across the genome. The technology combines the advantages of both karyotyping which enables the genome wide analysis for chromosomal imbalances and the high resolution of FISH. The resolution ...
Molecular karyotyping or genome wide array CGH enables the detection of chromosomal copy number variations across the genome. The technology combines the advantages of both karyotyping which enables the genome wide analysis for chromosomal imbalances and the high resolution of FISH. The resolution ...
Characterizing of Chromosomal Aberrations in Cancer Cells Genomic instability is the hallmark of cancer (Lengauer et al, 1998). As cancer cells develop, genomic aberrations accumulate. These aberrations include large DNA rearrangements such as chromosome loss or duplication or the translocation of ...
A lecture about Array-CGH and complex chromosome rearrangements by T.Pramparo from 2006
a lecture about Prenatal diagnosis and array-cgh by O. Zuffardi from 2008
Genomic changes are associated with altered gene expression that can have phenotypic consequences in cancer development and treatment. Genomic imbalances can be identified through measuring the ratios of fluorescence intensities following co-hybridisation of fluorescently labelled tumour and normal ...
Genomic changes are associated with altered gene expression that can have phenotypic consequences in cancer development and treatment. Genomic imbalances can be identified through measuring the ratios of fluorescence intensities following co-hybridisation of fluorescently labelled tumour and normal ...
Comparative genomic hybridisation (CGH) has become a widespread method for a genome wide analysis of DNA copy number changes in tumours. However, the detection of amplifications and deletions is limited when performing CGH to metaphase chromosomes. Replacing metaphase chromosomes as the hybridisatio ...
Comparative genomic hybridisation (CGH) has become a widespread method for a genome wide analysis of DNA copy number changes in tumours. However, the detection of amplifications and deletions is limited when performing CGH to metaphase chromosomes. Replacing metaphase chromosomes as the hybridisatio ...
Use of genotype arrays to discover genetic alterations in cancer
Affymetrix SNP Genotype arrays •10,000 SNPs encoded on single array •120,000 SNPs on 2 arrays •500,000 SNPs on 2 arrays (1 array) Major difference between SNP arrays and Oligo and BAC arrays is that each allele is interrogated separately. SNP arrays can detect abnormalities undetectable ...
Array Comparative Genomic Hybridization (array CGH) is the high-resolution laboratory technique of choice for the detection of chromosomal DNA copy number aberrations on a genome-wide scale [1;2]. To perform array CGH initially PCR amplified bacterial artificial chromosomes (BACs) or cDNAs were spot ...
Array -CGH is nowadays the method of choice for studying DNA copy number changes. However, the lack of commercial availability of the arrays is a disadvantage1. Until now, BAC and cDNA clones have been obtained from, for example, the Sanger Center, BACPAC Resources, InVitrogen and Research Genetics. ...
There have been recent exciting advances in fluorescence in situ hybridization (FISH) and array-based techniques which are changing the nature of cytogenetics both in basic research and in molecular diagnostics. With these new approaches, cytogenetic analysis now extends beyond the simple descriptio ...
There have been recent exciting advances in fluorescence in situ hybridization (FISH) and array-based techniques which are changing the nature of cytogenetics both in basic research and in molecular diagnostics. With these new approaches, cytogenetic analysis now extends beyond the simple descript ...

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Keywords

5s ribosomal rna, abnormal, affected, algorithm, allele, allele frequency, alpha, amniocentesis, amniotic fluid, Analysis of the entire coding region: Mutation scanning, Analysis of the entire coding region: Sequence analysis, aneuploidy, antigen, antigen binding fragment, antisense oligonucleotide, apoptosis, Array genomic hybridization, association, association studies, autism, autosomal dominant, autosomal recessive, b lymphocytes, bacterial artificial chromosome, balanced translocation, band, base, base pair, beta, bioinformatics, BRCA, cancer genetics, candidate gene, carrier, carrier testing, cell, cell culture, cell cycle, cell line, centromere, chi-square, chorion, chorionic villus sampling, chromatin, chromosomal analysis, chromosome, chromosome abnormality, cis configuration, clone, cohort, complement, Complementary DNA, conception, concordance, congenital, consanguinity, constitutional, copy number polymorphism, correlation, cosmid, coupling, cpg island, critical region, Cytogenetic Abnormality, Cytogenetic Analysis, cytogenetics, cytosol, de novo, degenerate oligonucleotide-primed pcr, deletion, deoxyribonucleic acid, diagnostic testing, differentiate, dna microarray, dna structure, domain, duplication, dysmorphic feature, dysmorphology, dysplasia, enhancer, epigenetics, ER, exon, expressed gene, expression, familial, fetus, fibroblast, fixed, Fluorescence in situ Hybridization, fluorescent in situ hybridization, frequency, gametogenesis, gene, gene expression, gene targeting, gene therapy, genetic counselling, genetics, genome, genomic dna, genomics, genotype, germ cell, gray, growth factor, helix, hereditary, heredity, heterogeneity, heterozygote, heterozygous, homozygote, homozygous, human leucocyte antigen, huntingtin, hybridization, immunohistochemistry, in vitro, in vivo, incomplete penetrance, index case, informative, informed consent, inherited, interstitial deletion, inv, inversion, isochromosome, isolate, isolated, karyogram, karyotype, kilobase, leucine zipper, ligation, linkage, locus, loss of heterozygosity, malformation, marker, maternal, meiosis, messenger rna, metabolism, metaphase, metastasis, methylation, methylation analysis, microarray, microdeletion, microsatellite, microtubules, mitosis, Molecular Analysis, molecular genetics, mosaic, mosaicism, Multicolor FISH, multivariate, mutant, mutation, new mutation, northern blot, nucleus, oligonucleotide, oncogene, paint, parental, penetrance, peptide, pericentric, phase, phenotype, point mutation, polymerase chain reaction, polymorphism, population, power, preimplantation diagnosis, preimplantation genetic diagnosis, prenatal diagnosis, prenatal screening, primer, proband, probe, prostate, protein, proteome, quantitative, quantitative PCR, radiation absorbed dose, rearrangement, recessive, reciprocal translocation, recombination, replication, ribonucleic acid, ribosomal RNA, rna interference, Robertsonian translocation, screening, segmental, segmental duplication, segregation, selection, sensitivity, sequence, single nucleotide polymorphism, Spectral Karyotyping, spindle, sporadic, stem cell, subtelomeric region, survival, syndrome, synthesis, t lymphocyte, t-cell, target dna, telomere, tetrasomy, threshold, transcript, transcription, transcription factor, transformation, translation, translocation, trisomy, trisomy rescue, tumor suppressor gene, ultrasound, unaffected, unbalanced translocation, uniparental disomy, variance, variation, vector, x-chromosome, y-chromosome, zinc finger

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